Skip to main content
. 2020 Feb 4;14(5):1154–1169. doi: 10.1038/s41396-020-0598-2

Fig. 4. Involvement of SK36 lipid metabolism and cell division genes in the elongated chain phenotype induced by Cd.

Fig. 4

a The expression of key SK36 FASII and cell division genes was quantified by qRT-PCR in SK36 grown to early exponential phase in Cd supernatants compared with the BHI control. b Bacterial chain morphology and (c) average chain length (μm) of the wild-type SK36, the deletion mutants (ΔgldA, ΔSSA_0278, and ΔSSA_0279), and the complemented (ΔgldAc, ΔSSA_0278c, and ΔSSA_0279c) strains after being treated with Cd supernatants (sup) compared with BHI. The pictures are representative of three independent experiments. Scale bars indicate 10 μm. d The expression of gldA in the wild-type and the ΔSSA_0278 and ΔSSA_0279 deletion mutant strains was compared in the presence of Cd supernatants (sup) versus BHI. Gene expression data are presented relative to the BHI control samples, which were arbitrarily assigned a value of 1. Data represent the means of three biological replicates. Error bars denote standard deviations.