Table 3.
Primer lists used in RT-qPCR reactions.
| Gene name | Gene description | Forward primer (5′-3′) | Reverse primer (5′-3′) | Product (bp) |
|---|---|---|---|---|
| SNZ2 | pyridoxal 5′-phosphate synthase | CAAGGATCTAGGTGAGGCTTTG | CTGGATCTCCGCCTTAATCTTG | 130 |
| THI21 | hydroxymethylpyrimidine kinase | ATTTGGCTCGCGGTTATTCC | GCCCGTTGTCCTTAACAGTC | 114 |
| THI6 | thiamine-phosphate diphosphorylase | TATGGCAATTGACGCCGATG | GCCAATGTCTCTACCTCCGA | 132 |
| THI80 | Thiamine pyrophosphokinase | ATCCATCCAAACGAGGATGA | CTATTGGCTGCACCATCTGC | 131 |
| ACT1 | Actin | ACATCGTTATGTCCGGTGGT | CCACCAATCCAGACGGAGTA | 142 |