Figure 1.

Oxidative stress response after a total dose of 2.2 J/cm². Male Wistar rats aged 7 weeks were exposed to GaN-on-GaN (GoG) LED or conventional white LED (XE) (Xanlite XXX Evolution 5 W) for 9 and 10 h respectively at 1900 lx, receiving the estimated retinal dose of 2.2 J/cm² (BLH weighted 0.253 J/cm² for regular LED and to 0.26 J/cm² for GaN-on-GaN LED). 15 hours after the end of the exposure period, the eyes were included in optimal cutting temperature medium (Tissue Tek), cryosectioned and immunostained. NE: Non exposed. (A) sections were stained with anti-Glial Fibrillary Acid Protein (GFAP) and (B) with anti-superoxide dismutase 2 (SOD2). Nuclei were stained in blue with DAPI. ONL indicates the outer nuclear layer, INL the inner nuclear layer and GCL the ganglion cell layer. Scale bar represents 100 µm. Photographs show representative images of the upper retina.