Fig. 3. Quantitative analyses of 16S rRNA and transcripts of hydABs, sqr, soxB, soxC, gyrB, and gap genes by RT-PCR.

a Agarose gel electrophoresis of PCR products for 16S rRNA, hydABs, sqr, soxBC, gyrB, and gap genes with or without RT of total RNA extracts from kAlv gills. b Agarose gel electrophoresis of PCR products for 16S rRNA, hydA1B1, sqr, soxBC, gyrB, and gap genes with or without RT of total RNA extracts from eAlv gills. c Comparative abundances of 16S rRNA gene in the DNA extracts from kAlv and eAlv gills by quantitative PCR analysis and of hydA1B1, hydA2B2, sqr, soxB, gyrB, and gap gene transcripts in the cDNA assemblages from kAlv and eAlv gills by quantitative RT-PCR analyses. Quantity of transcript (<1.7 × 10³ copies/g) of hydA1B1 gene in eAlv holobiont was lower than the detection limit by qPCR analyses. NA indicates not analyzed because the hydA2B2 gene was not detected by PCR in the eAlv endosymbiont.