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. 2020 Apr 21;10:6692. doi: 10.1038/s41598-020-63593-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Amine, Carboxyl, and PEG- modified particle effective diffusivity (Deff) on 21do, 5do, NEC-stressed (N-S), NEC-stressed and orally dosed with LYS (N-S-LYS), and NEC-stressed and orally dosed with DHA (N-S-DHA) tissue, at a time scale of τ = 1 sec. Data are presented as the average of at least 300 particles and error bars represent standard error of the mean of all tracked particles. ANOVA was used to determine significance between groups at *p < 0.05. (B) Representative particle trajectories that were used to calculate Deff. Scale bar: 10 µm. (C) Particle motion type was characterized as immobile (α: 0–0.2), subdiffusive (α: 0.2–0.8), or diffusive (α: 0.8–1.0) by fitting the log-log MSD plot to find the anomalous exponent, α. Data are presented as the average of at least 300 particles and error bars represent standard deviation of all tracked particles. ANOVA was used to determine significance at *p < 0.05 compared to immobile carboxyl- modified particles on 21do, ^#p < 0.05 compared to subdiffusive carboxyl- modified particles on 21do, ^p < 0.05 compared to diffusive carboxyl- modified particles on 21do.