Fig. 4.

NUSAP1 stabilizes ATR by sumoylation. a The level of ATR in cells with NUSAP1 knockdown. b The level of ATR in cells overexpressing NUSAP1. c The level of the indicated proteins in NUSAP1-knockdown U-87 MG cells treated with 100 µg/ml CHX for 0 h, 1 h, 2 h, 4 h, or 8 h. d The levels of the indicated proteins in NUSAP1-knockdown LN-229 cells treated with 100 µg/ml CHX for 0 h, 1 h, 2 h, 4 h, or 8 h. e The level of ubiquitinated ATR in the indicated GBM cells. f Interaction of endogenous NUSAP1 with endogenous ATR. g Interaction of exogenous NUSAP1 with exogenous ATR. h Diagram of different domains of NUSAP1. i, j Interaction of ATR with truncated NUSAP1 in 293FT cells. k Diagram of the SAP domain on the N-terminus of NUSAP1. l The level of ATR in NUSAP1-overexpressing cells and in NUSAP1-ΔSAP-overexpressing cells. m The expression of sumoylated ATR and ubiquitinated ATR in NUSAP1-overexpressing cells and in NUSAP1-ΔSAP-overexpressing cells, respectively