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. 2020 Apr 21;10:6751. doi: 10.1038/s41598-020-63290-3

Figure 1.

Figure 1

SOX3 expression is found within a subset of undifferentiated spermatogonia. (A) IF of adult testis cross sections showing expression of SOX3 is restricted to spermatogonia. Note the staining in Leydig cells is non-specific. (B) The number of SOX3 + spermatogonia remains constant, independent of the stage of seminiferous epithelium cycle, whereas CCND1 + spermatogonia are more prevalent in stage I-VI tubules. (C) IF of SOX3 and PLZF in P7 testis cross sections demonstrates an extensive overlap, with only a few SOX3-/PLZF + spermatogonia present. (D) 60X magnification of white box in (C) highlighting variations in PLZF expression from low (*) to high. (E) IF of SOX3 and GFRα1 in P7 testis cross-sections showing limited overlap of expression. (F) IF of SOX3, PLZF and SALL4 in cultures of undifferentiated spermatogonia derived from juvenile mice, PLZF and SALL4 are detected within essentially all cells, while SOX3 is only present within a subset of cells. Scale bars are 50μm. (A,C,E&F), 10μm (D).