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. 2020 Apr 21;10:6751. doi: 10.1038/s41598-020-63290-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Characterising the expression of SOX3 in adult testis. Assessing co-expression of SOX3 with a panel of undifferentiated and differentiated spermatogonia cell markers in adult seminiferous tubules by wholemount IF. SOX3 shows limited overlap in expression with stem cell-associated marker GFRα1 (A) but is co-expressed with progenitor markers RARγ (B) and LIN28A (C) within the undifferentiated pool. SOX3 is expressed at low levels or is undetectable in mid-late stage differentiating spermatogonia marked with c-KIT (D). Note that antibodies to SOX3 and GFRα1 are both raised in goat and detected in the same fluorescence channel. Cell staining patterns are used to distinguish GFRα1 (cell membrane) and SOX3 (nucleus). Selected undifferentiated cells and stages of tubules are indicated. Representative images are shown (n = 4 mice). Scale bars are 50μm.