Fig. 1. Phylogenomic comparisons of the novel euryarchaeal MAGs to other Diaforarchaea.

a Maximum-likelihood (ML) phylogenomic reconstruction of the MV2/SJ3-Eury MAGs and other Diaforarchaea. The analysis comprised 103 housekeeping protein homologs conserved across Archaea. Branch length is relative to the scale provided at the top indicating the expected number of substitutions per site. Bootstrap values >90 for nodes are indicated by black circles while those between 50 and 90 are denoted by white circles. Values are not depicted for nodes with bootstrap support <50 (out of 100 ML bootstraps). Archaeoglobus fulgidus and Methanobacterium formicicum were used as the outgroups (not shown). Clade-level triangles indicate the phylogenetic diversity within each group via side lengths that are proportional to the distances between the clade’s closest and furthest taxa. b Heatmap showing the pairwise average amino acid identities (AAI) between members of the major groups of Diaforarchaea that were used in the phylogenomic analysis in a. Groups are indicated by colored lines based on the legend at the bottom right. Pairwise AAI values are colored according to the scale on the right. The dendrogram was constructed based on hierarchical clustering of the pairwise average AAI values and the branch length is proportional to the scale at the bottom of the dendrogram.