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. 2020 Feb 17;14(5):1316–1331. doi: 10.1038/s41396-020-0611-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Unrooted phylogenetic reconstruction of a concatenated DsrAB alignment block (alignment length = 1230 positions, n = 1218 homolog pairs). The tree is displayed with an artificial midpoint-rooting visualization. Branches are identified according to major taxonomic/functional groups (following the classification scheme of [49]). Bootstrap values >90 for clade-level bifurcations are indicated by black circles while those between 50 and 90 are denoted by white circles. Values are not depicted for nodes with bootstrap support <50. Branch length is relative to the scale provided at the top indicating the expected number of substitutions per site. The clade-level triangles indicate the phylogenetic diversity within each group via side lengths that are proportional to the distances between the clade’s closest and furthest taxa. b Paralogous rooting of the DsrA (top) and DsrB (bottom) subunits using a subset of taxa (n = 62) from the major lineages shown in the more expansive phylogenetic analysis in a. Bootstrap values are depicted the same as in panel a. Branch length is relative to the scale provided at the left indicating the expected number of substitutions per site. The root is labeled where the DsrA/DsrB subunits are presumed to have originated by a gene duplication prior to expansion and radiation. The clade-level triangles indicate the phylogenetic diversity within each group via side lengths that are proportional to the distances between the clade’s closest and furthest taxa.