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. 2020 Apr 21;11:1921. doi: 10.1038/s41467-020-15593-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Basal view of follicle cells at stage 10A (S10A), with stochastic induction of mCD8GFP expression (magenta). F-actin is marked by phalloidin staining. A-P and D-V indicate the anterior-posterior and ventral-dorsal axes. Arrow heads indicate the extremities of supracellular F-actin fiber patterns. b, c Fourier analysis of F-actin distribution along the AP (b) and DV (c) axes. d Lines corresponding to the supracellular F-actin fiber patterns indicated in a. e Basal view of follicle clone cells co-expressing mCD8GFP and moeABD-mCherry. Arrow heads indicate filopodia that radiate from the medial-basal stress fiber network. f Basal view of two follicle clone cells in contact, one marked with mCD8GFP and the other with mCD8RFP showing filopodia interdigitating. g Cross-sections of cells along the two dashed lines shown in f: filopodia marked by mCD8RFP insert into the mCD8GFP-expressing cell (*, top panel); filopodia marked by mCD8GFP insert into the mCD8RFP-expressing cell (**, bottom panel). The z-axis indicates the basal to apical direction. h Left panel: close-up view of intercellular filopodia from follicle clone cells with stochastic expression of mCD8GFP (green). MyoII (red) is visualized by using a MyoII-mCherry construct. Right panels: cross-sections along the two dashed lines indicated in the left panel showing filopodia penetrating the actomyosin cortex of the neighboring cell. The z-axis indicates the basal to apical direction. i Left panel: close-up view of follicle cells marked by MyoII-mCherry (red) and E-caderin-GFP (endo E-cad, green). Right panels: cross-sections along the two dashed lines indicated in the left panel showing filopodia penetrating the actomyosin cortex of a neighboring cell. The z-axis indicates the basal to apical direction. j Left panel: basal view of follicle cells, labeled with moeABD-mCherry and MyoII-GFP. Right panels: moeABD and MyoII shown separately. Scale bars are 10 μm in a, e, f, 5 μm in h left panel, i left panel, g and j, 2 μm in h right panels and i right panels. The results shown in a, e–j have been successfully repeated from the at least four independent experiments.