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. 2020 Apr 21;11:1921. doi: 10.1038/s41467-020-15593-2

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Basal view of follicle cells at stage 10A (S10A): Cdc42-mCherry (red) and stochastic clone expression of mCD8GFP (green). b Basal views of follicle cell clones not expressing (top panels) and expressing (bottom panels) the Cdc42DN transgene marked by mCD8GFP coexpression. MyoII is visualized using a MyoII-mCherry construct (Sqh::Sqh-mCherry). c Average filopodia length per cell for the n individual follicle cells not expressing (control, only mCD8GFP-expressing) and expressing Cdc42DN. For each analyzed individual cell, all filopodia not <0.5 µm were measured (3–11 filopodia per cell for control and 2–7 filopodia per cell for Cdc42DN). Nine egg chambers were analyzed for control and five for Cdc42DN. p < 0.0001 by two-sided Mann–Whitney test. d Basal view of follicle cell clones not expressing (control, only mCD8GFP expressing, top panels) and expressing (bottom panels) the Cdc42DN transgene, marked by mCD8GFP coexpression. F-actin is marked by phalloidin staining. The results shown in a, b, d have been successfully repeated from the at least four independent experiments. e Schematic representation of the actomyosin distribution at the basal side of follicle cells in control and Cdc42DN-expressing backgrounds. f Relative (clone/wild type) MyoII and F-actin intensities in the n individual control (only mCD8GFP expressing) and Cdc42DN cells. p < 0.0001 by two-sided Mann–Whitney test. g Relative (central/lateral) distribution of MyoII and F-actin intensity in the n individual control (only mCD8GFP expressing) and Cdc42DN cells. p < 0.0001 by two-sided Mann–Whitney test. In c, f, g boxes extend from the 25th to 75th percentiles, the mid line represents the median and the whiskers indicate the maximum and the minimum values. h MyoII-mCherry intensity over time for a representative cell in the control (only mCD8GFP expressing), Cdc42DN and Rho1DN backgrounds. The average MyoII intensity in the control background is set to 1. i Percentage of oscillating cycle time periods in the n individual control (only mCD8GFP expressing) and Cdc42DN-expressing cells. Scale bars are 10 µm in a, b, d.