FIGURE 2.

Knockdown of Rab27b in C-MSCs via lentiviral shRNAs vector transduction. (A) qRT-PCR analysis of Rab27b in C-MSCs cells infected with lentiviral vectors encoding shRNA targeting Rab27b mRNA (sh-Rab27b-1, sh-Rab27b-2, sh-Rab27b-3 and sh-Rab27b-4) or negative control (sh-NC). β-Actin was used as endogenous control. Results are shown as mean ± SD (*p < 0.05 vs. sh-NC, n = 3). (B) qRT-PCR analysis of Rab27a and Rab27b in C-MSCs infected with sh-Rab27b1 + 2 (lentiviral vectors encoding sh-Rab27b1 and lentiviral vectors encoding sh-Rab27b2) or negative control (sh-NC). GAPDH was used as endogenous control. Results are shown as mean ± SD (****p < 0.0001 vs. sh-NC, n = 3). (C) Western blotting of Rab27b and Rab27a protein using β-actin as a loading control. (D) Immunofluorescent staining of Rab27b and Rab27a (red) in C-MSC^sh–NC and C-MSC^{sh–Rab27b1+2}; cell nuclei were counterstained with DAPI (blue) (scale bar = 20 μm).