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. 2020 Apr 15;8:209. doi: 10.3389/fcell.2020.00209

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Copyright © 2020 Jin, Shen, Su, Cai, Liu, Weintraub and Tang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) qRT-PCR analysis of genes related to mitochondrial fatty acid oxidation; TCA, tricarboxylic acid cycle; ETC, electron transport chain. The amount of mRNA was normalized using GAPDH. (n = 3–4). (B) Western blotting of H3k4me3 protein using TBP as a loading control (n = 3). (C) Mitochondrial membrane potential was measured by JC-1 staining and the images were obtained by fluorescent microscopy. Scale bars = 100 μm. The deltapsim is expressed as the ratio of red fluorescence to green fluorescence (n = 5–6). Results are shown as mean ± SD (NS, p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001).