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. 2020 Apr 15;11:397. doi: 10.3389/fpls.2020.00397

FIGURE 3.

FIGURE 3

hen1-8 ovules are compromised in pollen tube guidance. (A–D) Histochemical GUS staining of wild-type (A,C) or hen1-8 (B,D) pistils at 12 h after pollination (HAP) with ProLAT52:GUS pollen. Arrowheads point at an ovule that failed to attract pollen. (E–G) Aniline blue staining of wild-type (E) or hen1-8 (F–G) pistils at 48 HAP with wild-type pollen. Arrowheads point at ovules that did not develop as a result of fertilization failure. Two to three overlapping high-magnification images were taken for one pistil and overlaid with Photoshop (Adobe) to show the whole pistil (A,B,E,G). Numbers at the bottom of (A), (B), (C), (D) are quantification of targeted ovules out of total ovules. Results are means ± SD (n = 15). Bars = 100 μm for (A,B,E,G), 20 μm for (C,D,F).