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. 2020 Apr 15;11:397. doi: 10.3389/fpls.2020.00397

FIGURE 5.

FIGURE 5

Downregulating HEN1 in outer integuments mimicked hen1-8 in ovule development. (A–E) Representative silique from wild type (A), two lines of ProINO:amiR-HEN1 (B,C), and two lines of ProINO:HEN1-RNAi (D,E) transgenic plants. For some pistils, two to three overlapping high-magnification images were taken and overlaid with Photoshop (Adobe). (F) Quantification of seed set. Results are means ± SD (n = 15). Different numbers indicate significantly different groups (One-Way ANOVA, Tukey’s multiple comparisons test, P < 0.05). (G–N) CLSM of representative ovules at stage 3-VI from the #1 (G,H) or #2 (I,J) line of ProINO:amiR-HEN1, and #1 (K,L) or #2 (M,N) line of ProINO:RNAi-HEN1. Numbers on the bottom of each image indicate displayed ovules/total ovules examined. Dotted lines illustrate either outer integuments (oi) or inner integuments (ii). Cc, central cell; ec, egg cell; sg, synergid. Bars = 1 mm for (A,C,D); 500 μm for (B,E); 20 μm for (G,I,K,M,J,L); 10 μm for (H, N).