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. 2020 Apr 15;11:397. doi: 10.3389/fpls.2020.00397

FIGURE 7.

FIGURE 7

ARF6 and ARF8 are ectopically expressed in hen1-8 ovules. (A–D) Stage 3-IV wild-type (A,B) or hen1-8 (C,D) ovules labeled by the ARF6 antisense probe (A,C) or sense probe (B,D). (E–H) Stage 3-IV wild-type (E,F) or hen1-8 (G,H) ovule labeled by the ARF8 antisense probe (E,G) or sense probe (F,H). Funiculus of wild-type ovules whereas integuments of hen1-8 ovules show strong signals by the antisense probes. (I,J) Relative ARF6 (I) or ARF8 (J) abundance in mature ovules of wild type or hen1-8 by qRT-PCRs. GAPDH and TUBULIN2 were used as internal controls. Results shown are means ± SE (n = 3). Asterisks indicate significant difference (t-test, P < 0.01). Bars = 50 μm for whole views of pistils and 20 μm for close-ups of ovules.