Figure 2.

Bone marrow repopulation is not affected by MHC class-I mismatch or mCMV infection. HCT donors were of male sex for tracking the fate of transplanted hematopoietic cells (HC) by qPCR specific for the Y-chromosomal gene sry. Donor HC and progeny thereof were quantitated in the tibial bone marrow cell population. Total cell numbers were determined by pthrp-specific qPCR. Symbols indicate mice analysed individually. Note that for comparison of all four HCT groups (recall Figure 1A) data in the lower left panel were reproduced from own previous work on HvG-HCT (Seckert et al., 2011).