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. 2020 Apr 15;10:157. doi: 10.3389/fcimb.2020.00157

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Copyright © 2020 Holtappels, Schader, Oettel, Podlech, Seckert, Reddehase and Lemmermann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitation of GvH-reactive and viral epitope-specific liver-infiltrating CD8⁺ T cells after GvH-HCT. Liver-infiltrating CD8⁺ T cells were isolated from liver tissue (yield from 10 livers) of infected GvH-HCT recipients on day 20. Their functional activity was tested in an ELISpot assay based on IFNγ secretion in response to stimulation with BALB/c (upper panels) or BALB/c-H-2^dm2 (lower panels) MEF as target cells that were exogenously loaded with graded loading-concentrations of the indicated synthetic viral peptides to reveal cumulative avidity distributions. Effector cells responding at a certain peptide concentration include cells that respond to concentrations < the indicated concentration. Ø, no viral peptide loaded. Error bars represent 95% confidence intervals determined by intercept-free linear regression.