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. 2020 Apr 15;8:269. doi: 10.3389/fbioe.2020.00269

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Copyright © 2020 Dorrello and Vunjak-Novakovic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Removal of lung epithelium with preservation of functional vasculature. (A) Schematic representation of single lung de-epithelialization on EVLP (R lung control, L lung treated). (B) Histological analysis showing removal of lung epithelium in the respiratory zone (upper panel). Immunostaining showing removal of alveolar Type I cells (Aq5) and preservation of endothelial cells (vWF) (lower panel). (C) Endothelial viability showed by uptake of Ac-LDL by endothelial cells in de-epithelialized lung. (D) Preservation of vasoresponsiveness of de-epithelialized lung to Endothelin-1 and Treprostinil. (E) De-epithelialized rat lungs recellularized with CFSE-labeled human SAECs. Attachment of CFSE-labeled SAECs in the alveoli (higher magnification panels). Images reproduced with permission from Dorrello et al. (2017).