TABLE 5.
Metabolic activity exhibited by the cells present within the bacterial biofilms in the presence of different concentrations of E. gunnii and L. petersonii EOs.
| Metabolic activity of biofilm cells (% ± SD) | ||||||||
| 0.01 μg/mL | 0.02 μg/mL | 0.05 μg/mL | 0.1 μg/mL | 0.2 μg/mL | 0.5 μg/mL | 1.0 μg/mL | 2.0 μg/mL | |
| EC/EUC | 100 (±0) | 100 (±0) | 100 (±0) | 64.00 (±0.57) | 60.81 (±1.67) | 55.60 (±1.67) | 52.92 (±1.15) | |
| EC/LEPT | 100 (±0) | 100 (±0) | 100 (±0) | 78.55 (±1.67) | 63.91 (±1.15) | 55.15 (±1.67) | 48.24 (±0.57) | |
| LM/EUC | 100 (±0) | 100 (±0) | 100 (±0) | 100.0 (±0) | 67.34 (±1.15) | 60.35 (±1.67) | ||
| LM/LEPT | 100 (±0) | 100 (±0) | 100 (±0) | 95.67 (±0.57) | 95.06 (±0.57) | 83.56 (±1.15) | 50.64 (±1.67) | 44.30 (±0.57) |
| PC/EUC | 100 (±0) | 100 (±0) | 100 (±0) | 40.80 (±1.67) | 33.38 (±0.57) | 32.18 (±0.57) | 22.57 (±1.67) | |
| PC/LEPT | 100 (±0) | 100 (±0) | 100 (±0) | 43.62 (±0.57) | 25.48 (±0.57) | 23.32 (±1.67) | 16.98 (±0.57) | 16.09 (±0.57) |
| PSA/EUC | 100 (±0) | 100 (±0) | 100 (±0) | 94.35 (±0.57) | 83.34 (±1.67) | 77.74 (±1.67) | ||
| PSA/LEPT | 100 (±0) | 100 (±0) | 100 (±0) | 93.39 (±0.57) | 85.03 (±1.67) | 83.55 (±1.67) | ||
| SA/EUC | 87.54 (±0) | 72.10 (±0) | 66.05 (±0.57) | 64.32 (±1.67) | 63.21 (±1.67) | |||
| SA/LEPT | 98.41 (±0.57) | 94.38 (±0.57) | 92.64 (±1.15) | 91.54 (±1.67) | 91.24 (±1.15) | 90.89 (±0.57) | ||
Results are expressed as metabolic activity percent, calculated through the MTT test respect to the control (100% of metabolic activity). Results are the mean of three experiments ± SD. EC: E. coli; LM: L. monocytogenes; PC: P. carotovorum; PSA: P. aeruginosa; SA: S. aureus; EUC: E. gunnii EO; LEPT: L. petersonii EO.