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Accumulation of soluble sugars in apple callus under low-nitrogen treatment. (A) Fructose, (B) sucrose, (C) glucose, and (D) sorbitol contents in apple callus after nitrogen starvation treatment. Changes in expression of (E) MdSUSY2 and (F) MdSUSY3 under nitrogen-starvation stress. Data are the means of three replicates with SEs. Different letters indicate significant differences between treatments, according to one-way ANOVA and Tukey’s multiple range test (P < 0.05).
