FIG 3.

Mutations in SoxR are pleiotropic for resistance to different antibiotics. (A) Verification of the mutations enriched in the global regulator library for resistance to thiamphenicol at a 100-μg/ml concentration. (B) Evaluation of the extent of synergistic pleiotropy for the mutations in soxR conferring resistance to doxycycline for resistance to thiamphenicol at a 100-μg/ml concentration. (C) Evaluation of the extent of synergistic pleiotropy for all mutations in soxR conferring resistance to doxycycline for resistance to azithromycin at a 120-μg/ml concentration. The colored lines indicate the variants, and the black lines indicate wild-type E. coli.