TABLE 4.
Relative expression levels of 21 genes affected by CRP V140W in response to gentamicin
| Gene | Name of gene product | Fold change in expression determined by: |
|
|---|---|---|---|
| RNA-seq | Real-time qPCR | ||
| flgB | Flagellar basal body rod protein | −308.10 | −747.88 |
| fliA | RNA polymerase sigma factor for flagellar operon | −213.54 | −429.55 |
| motA | Chemotaxis protein | −138.11 | −592.22 |
| motB | Chemotaxis protein | −74.83 | −342.51 |
| flhD | Flagellar class II regulon transcriptional activator | −19.34 | −75.41 |
| flhC | Flagellar class II regulon transcriptional activator | −13.43 | −92.20 |
| tar | Methyl-accepting chemotaxis protein II | −164.92 | −403.57 |
| cheA | Two-component system, chemotaxis family, sensor kinase | −44.62 | −327.04 |
| pdeH | Cyclic-di-GMP phosphodiesterase | −107.17 | −367.94 |
| flu | CP4-44 prophage antigen 43 phase-variable biofilm formation autotransporter |
−12.74 | −45.78 |
| leuB | 3-Isopropylmalate dehydrogenase | 41.74 | 1.64 |
| leuA | 2-Isopropylmalate synthase | 40.36 | 1.31 |
| serC | 3-Phosphoserine/phosphohydroxythreonine aminotransferase | 8.28 | 1.30 |
| tdcB | l-Threonine dehydratase | −448.77 | −11,036.54 |
| fadB | 3-Hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase/enoyl-CoA isomerase | −21.58 | −167.73 |
| yqeF | Beta-ketoacyl-CoA thiolase | −6.72 | −52.35 |
| tktB | Transketolase B | 36.99 | 3.46 |
| talA | Transaldolase A | 28.78 | 5.46 |
| pfkA | 6-Phosphofructokinase I | −89.48 | −8,079.22 |
| sdhC | Succinate dehydrogenase | −27.10 | −307.26 |
| mqo | Malate dehydrogenase | −6.49 | −73.18 |