TABLE 2.
MSMEG genes affected by zinc excess
| RCNa | Annotationc | Orthologous gene (% similarity) |
q valued | Fold changee | Putative functionf | |
|---|---|---|---|---|---|---|
| MTB | MAP | |||||
| —b | MSMEG_0230 | Rv0190 (80.0) | MAP3632 (85.9) | <0.0001 | 5.29 | Hypothetical protein |
| — | MSMEG_0689 | — | — | <0.0001 | −4.04 | Hypothetical protein |
| — | MSMEG_0755 | Rv0359 (68.8) | MAP3865c | <0.0001 | 14.36 | Cobalt-zinc-cadmium resistance protein |
| — | MSMEG_1392 | — | — | <0.0001 | 5.37 | Alcohol dehydrogenase |
| — | MSMEG_1530 | — | — | <0.0001 | −4.26 | Integral membrane protein |
| — | MSMEG_3323 | — | MAP0103c (80.2) | <0.0001 | 4.80 | Hypothetical protein |
| — | MSMEG_3325 | — | MAP0102 (91.0) | <0.0001 | 5.38 | Hypothetical protein |
| — | MSMEG_5014 | Rv0969 (52.8) | MAP4284 (63.6) | <0.0001 | 6.25 | Zinc/cadmium/cobalt P-type ATPase |
| — | MSMEG_5015 | — | — | <0.0001 | 10.36 | Hypothetical protein |
| — | MSMEG_5016 | — | — | <0.0001 | 6.53 | Hypothetical protein |
| — | MSMEG_5117 | Rv1188 (68.3) | MAP2592c (71.6) | <0.0001 | 11.75 | Proline dehydrogenase |
| — | MSMEG_5418 | — | — | <0.0001 | −4.46 | Iron permease |
| — | MSMEG_5549 | Rv0943c (51.0) | MAP0887c (71.9) | <0.0001 | 4.63 | Hypothetical protein |
| — | MSMEG_6237 | — | — | <0.0001 | −11.22 | Hypothetical protein |
| — | MSMEG_6242 | — | — | <0.0001 | −4.64 | Alcohol dehydrogenase → oxidative stress |
| — | MSMEG_6292 | — | MAP1027c (41.9) | <0.0001 | 4.53 | Transcription elongation factor GreA |
| — | MSMEG_6664 | — | — | <0.0001 | 4.17 | Methylenetetrahydrofolate reductase |
| — | MSMEG_6764 | — | MAP0155 (43.2) | <0.0001 | 4.87 | TetR family transcriptional regulator |
| — | MSMEG_6903 | Rv0047c (91.9) | MAP0061c (88.8) | <0.0001 | −5.50 | PadR family transcriptional regulator |
| — | MSMEG_6904 | Rv0046c (85.7) | MAP0060c (86.8) | <0.0001 | −4.58 | Myo-inositol-1-phosphate synthase |
a
RCN, reference common name.
b
—, no reference common name/orthologous gene.
c
Italics indicate genes organized in an operon.
d
q value of differentially expressed genes in MSMEGwt standard culture versus MSMEGwt ZnSO4 culture calculated by Rockhopper analysis. A q value of <0.01 is considered significant.
e
Gene expression values of MSMEGwt ZnSO4 culture divided by gene expression values of MSMEGwt standard culture from RNA-Seq.
f
Putative function based on NCBI blastx analysis or the TB database.