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. 2020 Apr 15;11:610. doi: 10.3389/fimmu.2020.00610

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Copyright © 2020 Hou, Jiang, Ma, Zou, Piao, Liu and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Antigenicity of repetitive peptides. (A) The prevalence rates of antibodies recognizing different peptides with the repetitive motifs were derived from the microarray profile. The 30 types of repetitive sequences were represented in rows in ascending order of the average PR of all the peptides containing the motif (Mean ± SD). - indicates varied amino acid. The red dotted line indicates the 50% prevalence rate. (B,C) Thirteen extra synthetic peptides were printed onto the microarray chips and detected by sera from African FM patients. (B) Influence of the adjacent amino acids on the immunoreactivity of peptides with the G(D)ESKET motif (C) The number of G(D)ESKET required for eliciting optimal humoral responses. The prevalence rate for appropriate group (columns, up X axis) and the reaction intensity with the third quartile of signal noise ratio (Q3, red dot, low X axis) were showed.