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. 2020 Apr 15;11:358. doi: 10.3389/fgene.2020.00358

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Copyright © 2020 Ryan, Nasamran, Pak, Draf, Fisch, Webster and Kurabi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) PCA clusters generated from the cells of all three mucosal samples, generated with Seurat. Seventeen clusters (0–16) were identified. Marker genes were again used to determine the cells types present in each cluster. Some cell types that were grouped in contiguous clusters by Cellranger were separated in the Seurat analysis. Marker gene expression in: (B) Epithelial cell clusters (0, 1, 2, 6, 11); (C) Stromal cell clusters (3, 4, 5); (D) Vascular cell clusters (7, 12, 13); (E) Monocyte clusters (8, 9); and (F) Lymphocyte clusters (10, 14, 15).