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. 2020 Apr 15;8:227. doi: 10.3389/fcell.2020.00227

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Copyright © 2020 Georgatzakou, Pavlou, Papageorgiou, Papassideri, Kriebardis and Antonelou.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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((A) Percentage of research and review articles dealing with plasma EVs in CKD published in the last decade (N = 56). (B) The vast majority of research data on plasma EVs in CKD derived in the period from 2002 till today (N = 37) were based exclusively on flow cytometry (FC). Fewer studies combined FC with other methodologies (immunoblotting, nanoparticle tracking analysis, proteomics, Elisa, electron microscopy) or they did not use FC at all. References are shown in the Supplementary Material.