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. 2020 Apr 15;11:611. doi: 10.3389/fimmu.2020.00611

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Copyright © 2020 Motwani, Peters, Vliegen, El-sayed, Seay, Lopez, Baker, Posgai, Brusko, Perry, Bacher, Larkin, Haller and Brusko.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bulk gene-expression profiles from expanded cells show increased regulatory phenotype in both Tregs and Tconv from CB compared to APB. (A) Volcano plots showing log2 (Fold Change) versus -log10 (adjusted p-value) annotated with differentially expressed genes (155) colored by downregulation (blue) and upregulation (red). (B) Heatmap of canonical Treg and Tconv genes shows clustering by cell type (Treg (n = 13) versus Tconv (n = 13) and also sample origin (APB (n = 14) versus CB (n = 12).