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. 2020 Apr 15;11:611. doi: 10.3389/fimmu.2020.00611

FIGURE 5.

FIGURE 5

Expanded APB Tregs and CB Tregs have similar cytokine production profiles. Expanded CB Tregs (blue, n = 4), APB Tregs (orange, n = 8), CB Tconv (green, n = 3), and APB Tconv (red, n = 3) were activated with PMA/ionomycin and cytokine production detected by Luminex assay. CB Tregs and APB Tregs displayed limited differences in cytokine production profile, as there were no differences in production of (A) IL-20, (B) IL-22, (C) IL-26, (D) IL-27, (E) IL-12p40, (F) IL-12p70, (G) IL-28, or (H) IL-29. APB and CB Tregs produce similar amounts of immunosuppressive cytokines (I) IL-19, (J) IL-35, and (K) IL-10, while APB Tregs produced significantly more (L) IL-2 relative to CB Tregs. When comparing CB Tconv versus APB Tconv, (A–J,L) cytokine production was similar except APB Tconv produced significantly more (K) IL28A/IFNg2 as compared to CB Tconv. Data were analyzed in R with a mixed effects model using the lmer function in the lme4 package, as noted in Materials and Methods. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.