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. 2020 Apr 15;11:622. doi: 10.3389/fimmu.2020.00622

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Copyright © 2020 Tector, Mosser, Tector and Bach.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of carbohydrate epitopes in genetically modified pigs (54). Confocal microscopy of 2-month-old WT, 8-month-old GGTA1 KO, and 2-day-old GGTA1/CMAH double-KO porcine tissues stained with (A) anti-Neu5Gc chicken IgY (cyan, Sialix, Vista, CA, United States) and (B) IB4 lectin (red). (C) Flow cytometric analysis of PBMCs labeled with anti-Neu5Gc antibody (blue) and IB4 lectin (red). Unstained PBMCs were the negative controls for IB4 lectin, and an isotype negative control was used in the anti-Neu5Gc staining. Although shown, the negative controls are difficult to see in some panels because of overlap with the experimental group.