Skip to main content
. 2020 Apr 15;11:622. doi: 10.3389/fimmu.2020.00622

FIGURE 2.

FIGURE 2

Comparison of human antibody binding to human, porcine or chimpanzee cells (55). PBMCs isolated from humans, chimpanzees and GGTA1/CMAH KO pigs incubated with 25% serum collected from five humans. Levels of IgM or IgG binding were detected using fluorescently labeled anti-human IgM or IgG antibodies followed by flow cytometry analysis. Histogram profiles of human IgM and IgG antibody binding are shown for three humans (filled gray), three chimpanzees (black) and three GGTA1/CMAH KO pigs (red). Histogram profiles of PBMCs incubated with fluorescently labeled anti-human IgM or IgG antibodies in the absence of human serum are shown to indicate background fluorescence (2° only). CMAH, cytidine monophosphate-N-acetylneuraminic acid hydroxylase; GGTA1, galactosyltransferase.