Figure 7.

Roflupram (ROF) or rolipram (Roli) reduce the loss of TH in the SN of MPTP‐treated mice. (a) Nissl staining of the SN of mice in different samples under the microscope. (b) Number of Nissl‐positive cells, as exhibited in (a). (c) Immunohistochemistry of the SN of mice. (e) Number of TH‐positive cells, as exhibited in (c). (d) Tissue immunofluorescent staining of the SN of mice. Scale bar = 200 μm. (f) The SN of each mouse was analysed and the expression of TH was measured by western blotting. (g) Densitometric quantification of TH/GAPDH in (f). Data are presented as mean ± SD (n = 5) and represent five independent experiments. ^# P < .05, significantly different from vehicle group. *P < .05, significantly different from MPTP‐treated group