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. 2019 Dec 6;30(4):2114–2127. doi: 10.1093/cercor/bhz226

Figure 2.

Figure 2

DAB blockade of the LTP-dependent increase in spine formation and stabilization are rescued by l-lactate. (A) Individual spines on dendritic segments are imaged in transfected CA1 hippocampal pyramidal neurons. (B) Cch increased the percentage of eliminated spines (lost spines) at 24 hours after inducing of LTP in all experimental conditions. (C) Cch-induced LTP increase of the percentage of new spines after 24 hours was blocked with DAB and rescued by l-lactate. (D) Preexisting individual spines (left, white arrowhead) were either enlarging at 5 hours and stabilized over 4 days (yellow arrowhead) or nonenlarging at 5 hours (gray arrowhead). (E) Cch-induced selective spine stabilization was blocked by DAB and rescued by l-lactate. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. (B), (C) One-way analysis of variance, Kruskal-Wallis test. (E) Two-way analysis of variance. Scale bars: (A) left: 500 μm, right: 50 μm, bottom: 4 μm, (D) 2 μm (see Table S2).