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. Author manuscript; available in PMC: 2020 Apr 22.
Published in final edited form as: Cell Rep. 2020 Mar 24;30(12):4250–4265.e6. doi: 10.1016/j.celrep.2020.03.004

Figure 1. Single-Cell Evaluation of Native Rat Tracheal Epithelium.

Figure 1.

(A) Uniform Manifold Approximation and Projection (UMAP) of epithelial cell clusters.

(B) Heatmap of top DEGs for each cluster (lowest p value, see Method Details); columns grouped by cluster representing 60 randomly selected cells per cluster to show cluster heterogeneity; rows represent unity normalized expression of top 8 DEGs per cluster.

(C) Diagram of native rat tracheal epithelium, color-coded to UMAP clusters.

(D) IF of key markers for each cluster, including KRT5/IGFBP2 (BCs), CCSP/SPD (secretory; red background staining in lamina propria), DCLK1(tuft)/Tub1α(ciliated), and MUC19 (SMG secretory) (scale bars, 25 μm).

See also Figure S1.