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. Author manuscript; available in PMC: 2020 Apr 22.
Published in final edited form as: Cell Rep. 2020 Mar 24;30(12):4250–4265.e6. doi: 10.1016/j.celrep.2020.03.004

Figure 3. peBCs Achieve Robust Proximal Epithelial Differentiation in 3 Platforms and More Distal Character in Engineered Lung.

Figure 3.

(A–D) peBC differentiated as (A) organoid, (B) ALI, (C) engineered trachea, and (D) engineered lung.

(A) Bright field of organoids at 2 weeks in Matrigel (scale bar, 100 μm).

(B) SEM of ALI at 3 weeks (scale bar, 10 μm).

(C) H&E of engineered trachea at 4 weeks (scale bar, 50 μm).

(D) Stitched H&E image and inset of the upper right lobe of engineered lung at 1 week taken with EVOS system (scale bars 750 μm, 50 μm, resp.); IF of KRT5/ MUC5AC in alveolar region; KRT5/MUC5AC and KRT5/Tub1α in the trachea showing a lack of secretory and ciliated cells (scale bars, 25 μm); IF of AQP5 in alveolar region, CCSP in bronchiole (scale bars, 50 μm).

In (A)–(C), IF of KRT5/MUC5AC and KRT5/Tub1α in organoid, ALI and engineered trachea; organoid lumens and apical cell surfaces oriented up (scale bars, 25 μm).

See also Figures S3–S5.