FIG 1.

Inhibition of the proteasome induces C. albicans morphogenesis. (A) Genetic depletion of proteasome subunits of the 19S regulatory particle or 20S core particle induces morphogenesis in the absence of external inducing cues. Cells were grown in YPD medium in the presence of 0.05 μg/ml DOX at 30°C, with shaking, for 24 h to repress target gene expression. Strains were grown in batches with the wild-type control. Blue and red indicate proteasome subunit genes that are essential and nonessential for growth, respectively. (B) Pharmacological inhibition of the proteasome with two structurally distinct drugs, MG132 (200 μM) and bortezomib (520 μM), induces morphogenesis. Strains were grown in the absence or presence of drug at 30°C for 24 h under static conditions. Scale bar, 10 μm.