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All three CFEM hemophores extract heme from HSA. HSA (10 μM) was preloaded with 5 μM hemin in a spectroscopy cuvette, and then the indicated CFEM protein was added to 10 μM, and absorption spectra were taken every minute for 90 min. The inset shows the change in absorbance with time, at the wavelength that distinguishes best between heme bound to HSA and heme bound to the relevant CFEM protein.
