Skip to main content
. 2020 Mar 19;10(3):468. doi: 10.3390/biom10030468

Figure 3.

Figure 3

Schematic representation of nuclear pore complex (NPC) biogenesis highlighting the two distinct assembly mechanisms. Post-mitotic insertion occurs during late mitosis where pre-existing NPC subcomplexes assemble at the reforming NE. Interphase assembly requires the de novo construction of NPCs into the double lipid bilayer of the NE. Upon Torsin manipulation, interphase assembly is stalled presumably prior to the fusion of the inner nuclear membrane (INM)/outer nuclear membrane (ONM). Note that cytoplasmic fibrils (CFs) containing NUP358 are only added to the nascent NPC after INM/ONM fusion during interphase assembly (i.e., the observed absence of NUP358 from blebs containing NPC components supports the idea of blebs representing stalled NPC assembly intermediates) [32,84].