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. 2020 Mar 3;10(3):392. doi: 10.3390/biom10030392

Figure 5.

Figure 5

ER stress or IRE1α inhibition impair Zn (II)-curc-induced cell death. (a) Cell viability was measured by trypan blue exclusion assay in U373 and HT29 cells untreated or treated with Zn (II)-curc (100 µg/mL) for 24 h, with or without the inhibitor of XBP1 cleavage STF (60 μM), p53 inhibitor PTF-α (30 μM), or 1 h pre-treatment with 4-BPA (2.5 mM), and expressed as percentage ± SD of three independent experiments. * p ≤ 0.05 (combination treatments vs. Zn(II)-curc). (b) Cytofluorimetric analysis of the SubG1 peak evaluated by Propidium Iodide (PI) staining of U373 and HT29 cells untreated or treated as in (a) for 24 h. Percentage of apoptotic cells is shown ± SD of two independent experiments. (c) U373 and HT29 cells were transfected with control pSuper (si-ctr) and pSuper-p53 (si-p53) vectors for p53 knockdown and 36 h after transfection, cells were treated with Zn(II)-curc (100 µg/mL) for 24 h. Cell viability was measured by a trypan blue exclusion assay and expressed as percentage ± SD of three independent experiments. * p ≤ 0.05.