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. 2020 Mar 20;10(3):473. doi: 10.3390/biom10030473

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A). Biochemical isolation of GJA1-11k in the nucleus. Western Blot of Cx43 isoforms in subcellular fractions (cytosolic and nuclear fractions) of HEK293FT cells over-expressing exogenous GJA1-11k, GJA1-43k, and GFP-V5 tagged plasmid cDNA, probed to monoclonal Cx43-CT (Millipore) antibodies. To demonstrate enriched biochemical isolation, the same blot was probed using antibodies to different subcellular fraction markers, including cytoplasmic (GAPDH), membrane (Na⁺/K⁺-ATPase), Golgi (GM-130), and nuclear markers (Histone H3, Lamin B1). The results are representative of three independent experiments. (B). The densitometry of CT-43k (11k) subcellular fractions assessed by Western Blot. Uncut immunoblots are provided in Figure S3.