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. 2020 Mar 20;10(3):473. doi: 10.3390/biom10030473

Figure 4.

Figure 4

Effect of Cx43 short isoforms on cell proliferation. The overexpression of GJA1-11k inhibits the cell growth of the HEK293FT cell line. The growth curve is counts of transiently transfected cells each with vectors expressing GJA1-11k, GJA1-43k, GJA1-WT, and the negative control plasmid GFP with V5-tag. For each day data are presented as mean ± SEM, n = 12, ** p < 0.01 by one-way ANOVA followed by Tukey’s post-hoc test. Cell growth analysis revealed that cells expressing GJA1-11k grew significantly slower, than cells overexpressing other isoforms. Western Blot confirmed transient expression of Cx43 isoforms. Uncut immunoblots are provided in Figure S6.