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. 2020 Feb 28;10(3):371. doi: 10.3390/biom10030371

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Denaturation profiles of bovine and porcine pericardia before and after TRICOL decellularization. (A) Second derivatives of the recorded spectra between 1600 and 1700 cm⁻¹. No modifications were induced by TRICOL decellularization on the secondary structure, i.e., α-helical structures (1650 cm⁻¹) and ß-sheets (1630 cm⁻¹). (B) R ratio. R was not significantly modified by TRICOL decellularization in both species’ tissues. (C) Differential scanning calorimetry (DSC) thermograms. Similar profiles were observed when decellularized and native pericardia of both species were compared. Glutaraldehyde treatment sensibly modified tissue thermograms. (D) The onset temperature (T_onset) of protein denaturation. Denaturation occurred at decreased temperature with respect to native tissues only for porcine pericardium. Glutaraldehyde treatment caused a significant increase of the denaturation temperature for both species with respect to all other groups considered. Data are presented as mean ± standard deviation (SD). *p <0.05, ***p <0.001. NBP= Native Bovine Pericardium, DBP= Decellularized Bovine Pericardium, NPP= Native Porcine Pericardium, DPP= Decellularized Porcine Pericardium, and GA= Glutaraldehyde.