Figure 8.

Tannic acid and derivatives protect endothelial cells from hypoxia–reoxygenation injury. (A) Cytotoxicity induced by the hypoxia–reoxygenation sequence was prevented by tannic acid (AT) and its main metabolites gallic acid (GA) and penta-O-galloyl-beta-D-glucose (PGG) with a dose-dependent response. Molecules were added in the culture medium during the hypoxia–reoxygenation sequence. n = 3, n = 2. (B) XTT analysis showing that cytotoxicity induced by the hypoxia–reoxygenation sequence was prevented by tannic acid (12.5 mg/mL, 7.3 µmol/L). Molecules were added in the culture medium during the hypoxia–reoxygenation sequence. n = 3, n = 2, values are mean ± SD, * p < 0.05 to the PBS condition (vehicle).