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. 2020 Mar 10;10(3):429. doi: 10.3390/biom10030429

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Histological sections from zebrafish testicular explants incubated for 7 days (long-term exposure) with 100 ng/mL cortisol compared with control (basal). (A) Basal (0 ng/mL). (B) Cortisol 100 ng/mL. Several germ cell types are indicated, such as type A undifferentiated spermatogonia (A_und); type A differentiated spermatogonia (A_diff); type B spermatogonia (B), spermatocytes (SPC), and spermatozoa (SPZ). (C) Histomorphometrical analysis of testicular explants incubated for 7 days with cortisol (100 ng/mL) in the absence or presence of 1 µg/mL RU 486 (glucocorticoid antagonist), compared to the control (basal). Bars (mean ± SEM; n = 8) represent the percentage of the spermatogenic cysts at different stages of germ cell development: A_und; A_diff; B; SPC and spermatids (SPT). (D) Spermatozoa quantification per field generated by using IMAGE J Software from zebrafish explants incubated for 7 days with either basal (L-15), cortisol (100 ng/mL), or cortisol (100 ng/mL) + RU 486 (1 µg/mL). ANOVA followed by Student–Newman–Keuls; different letters indicate significant differences (p < 0.05) between different treatment conditions.