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. 2020 Mar 10;10(3):429. doi: 10.3390/biom10030429

Figure 4.

Figure 4

BrdU immunodetection from zebrafish testicular explants incubated for 7 days (long-term exposure) with 100 ng/mL cortisol compared with control (basal). (A) Basal. (B) Cortisol 100 ng/mL. Type A undifferentiated spermatogonia (Aund); type A differentiated spermatogonia (Adiff); type B spermatogonia (B) and spermatozoa (SPZ). (C) BrdU labeling index of Aund, Adiff, and B in zebrafish testis treated in the absence (basal) or presence of cortisol (100 ng/mL) for 7 days of culture. Bars represent the mean ± SEM (n = 8). Paired t-test, *** p < 0.001; ** p < 0.01.