Table 1.
Primer sequences of flavonoid and chlorogenic acid biosynthetic pathways genes.
| Primer Name | Abbreviation | Sequence (5’-3’) | |
|---|---|---|---|
| β-actine | β-actine | F | GTGGGCCGCTCTAGGCACCAA |
| R | CTCTTTGATGTCACGCACGATTTC | ||
| Phenylalanine ammonia-lyase 1 | PAL1 | F | ACGGGTTGCCATCTAATCTGACA |
| R | CGAGCAATAAGAAGCCATCGCAAT | ||
| Cinnamic acid 4-hydroxylase | C4H | F | CCCAGTTTTTGGAAATTGGCTTCA |
| R | GCCCCATTCTAAGCAAGAGAACATC | ||
| Chalcone synthase | CHS | F | CACCGTGGAGGAGTATCGTAAGGC |
| R | TGATCAACACAGTTGGAAGGCG | ||
| Chalcone isomerase 2 | CHI2 | F | GGCAGGCCATTGAAAAGTTCC |
| R | CTAATCGTCAATGATCCAAGCGG | ||
| Flavanone 3-hydroxylase | F3H | F | CCAAGGCATGTGTGGATATGGACC |
| R | CCTGGATCAGTATGTCGTTCAGCC | ||
| Flavonol synthase 1 | FLS1 | F | CCTCCTTCCTACAGGGAAGCAAA |
| R | CAAGCCCAAGTGACAAGCTCCTAA | ||
| Dihydroflavonol 4-reductase | DFR | F | TCACAGGAGCAGCTGGATTTATCG |
| R | TCAGGATCACGAACAGTAGCATGG | ||
| Flavonoid 3′ hydroxylase | F3′H | F | TGGGTATACCCAAACTCATTCCG |
| R | AAAAGCCCAAAGTTGATGTGAAAGG | ||
| Anthocyanin 1 transcription factor | AN1 | F | CCTCAACCTCAGAAATTCAGAAGC |
| R | TCGTTGTTGTTGTCGTTCGATGC | ||
| Anthocyanin 2 transcription factor | AN2 | F | ACAAGATGCCACTTTCCTTCACC |
| R | TGTGCATCGTTGGGAGTTAGG | ||
| Hydroxycinnamoyl Co A shikimate hydroxycinnamoyl transferase | HCT | F | TCTCCAACCCCTTTTAACGAACC |
| R | CAACTTGTCCTTCTACCACAGGGAA | ||
| Hydroxycinnamoyl Co A quinate hydroxycinnamoyl transferase | HQT | F | CCCAATGGCTGGAAGATTAGCTA |
| R | CATGAATCACTTTCAGCCTCAACAA | ||
| p-coumarate 3-hydroxylase | C3H | F | TTGGTGGCTACGACATTCCTAAGG |
| R | GGTCTGAACTCCAATGGGTTATTCC |