Figure 5.
Bactericidal activity of the modified endolysins. (A) Activity of the endolysins against A. baumannii Ts 50-16, diluted in a PBS buffer solution (protein concentration of 1 µg/mL); (B) activity of endolysins against A. baumannii Ts 50-16, diluted in human serum (protein concentration of 10 µg/mL); (C) activity of lysins against the stationary-phase cells of A. baumannii Ts 50-16, diluted in 20 mM Tris HCl buffer pH 7.5 (protein concentration of 10 µg/mL). Culture dilution was 1 to 3 × 105 CFU/mL. For all experiments, the mean values are shown from three independent experiments ± SD. An asterisk (*) indicates a significant effect on bactericidal activity compared to the LysECD7 enzyme (p < 0.05, one-way ANOVA, Dunnett’s multiple comparisons test).