Figure 4.

CoCl₂ prevents SIRT1 nuclear translocation in microglia. In (a), Western blot analysis of SIRT1 expression in BV2 cells exposed to CoCl₂ (250 μM for 8 h) alone, or in combination with either melatonin (1 μM) or melatonin + EX527 (5 μM). In (b), immunostaining of SIRT1 (green) and nuclear counterstaining with DAPI (blue) in BV2 cells treated with CoCl₂ alone or in combination with melatonin (1 μM) or melatonin + EX527 (5 μM). Bars are mean ± SEM of three independent experiments. Representative images are shown. Scale bar = 20 μm.