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. 2020 Apr 15;11:430. doi: 10.3389/fpls.2020.00430

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Copyright © 2020 Sánchez-Simarro, Bernat-Silvestre, Gimeno-Ferrer, Selvi-Martínez, Montero-Pau, Aniento and Marcote.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of amiR-β1/β2-cop mutants. (A) RT-qPCR analysis to show the silencing of β1-COP and β2-COP in the amiR-β1/β2-cop lines. Total RNA was extracted from 4 days seedlings of the amiRNA lines amiR-β1/β2-cop-3, amiR-β1/β2-cop-5, amiR-β1/β2-cop10 and wild type (Col-0). The mRNA was analyzed by RT-qPCR with specific primers and normalized to UBQ10 expression (Supplementary Table S2). Results are from three biological samples and three technical replicates. mRNA levels are expressed as relative expression levels and represent fold changes of mutant/wild type. Values represent mean ± s.e.m of the three biological samples. (B) Phenotypes of amiRNA lines and wild type (Col-0) plants. Line 5: 36 day-old plants. Line 3: 26 (lower panel) and 50 day-old-plants (upper panel). Line 10: 26 (lower panel) and 36 day-old plants (upper panel).